Differentiation of EDTA-sensitive from EDTA-insensitive human serum esterases hydrolysing phenylacetate
نویسندگان
چکیده
منابع مشابه
Injurious effect of EDTA contamination on colorimetry of serum iron.
Colorimetry of iron in serum with Ferrozine (as used in the Technicon SMAC) or with bathophenanthroline (as used in the Du Pont aca) is influenced by EDTA, in contrast to such measurements with atomic absorption spectroscopy. Therefore EDTA contamination should be avoided with these colorimetric methods. If, however, contamination with EDTA is suspected, addition of zinc sulfate to serum or to ...
متن کاملEDTA-resistant and sensitive phosphotriesterase activities associated with albumin and lipoproteins in rabbit serum.
Phosphotriesterase (PTE) activities in mammalian serum are typically found in the lipoprotein fraction. This PTE requires Ca++ for activity and is consequently inactivated by ethylenediaminetetraacetic acid (EDTA). There is also a little known PTE in mammal serum that is resistant to EDTA inactivation. In this work, the PTE activities for the substrates O-hexyl O-2, 5-dichlorophenyl phosphorami...
متن کاملPurification and characterization of EDTA monooxygenase from the EDTA-degrading bacterium BNC1.
The synthetic chelating agent EDTA can mobilize radionuclides and heavy metals in the environment. Biodegradation of EDTA should reduce this mobilization. Although several bacteria have been reported to mineralize EDTA, little is known about the biochemistry of EDTA degradation. Understanding the biochemistry will facilitate the removal of EDTA from the environment. EDTA-degrading activities we...
متن کاملEDTA Chelation Therapy
This study measured excretion of 11 minerals induced by EDTA chelation therapy in 25 adults (aluminum, cadmium, calcium, chromium, copper, iron, lead, magnesium, manganese, mercury, zinc). We administered 20 EDTA infusions (3 g, with magnesium and ascorbate) 1 to 2 weeks apart and measured 24-hr urinary excretions of minerals before treatment and after treatments 1, 10, and 20. A modest supplem...
متن کاملEDTA-dependent lymphocyte clumping.
A 79-year old man presented with chest pain and fatigue. He was found to have anemia (hemoglobin 8.8 g/dL) and leukocytosis (white blood cell count 244x10/L) with a predominance of lymphocytes (94%). Flow cytometric study and flourescence in situ hybridization analysis of the peripheral blood lymphocytes were consistent with a diagnosis of mantle cell lymphoma. A peripheral smear obtained from ...
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ژورنال
عنوان ژورنال: Journal of Enzyme Inhibition and Medicinal Chemistry
سال: 2008
ISSN: 1475-6366,1475-6374
DOI: 10.1080/14756360701631637